黄河三角洲北部互花米草盐沼湿地区与贝壳沉积区微生物群落的DGGE分析

Analysis on microbial diversity in Spartinaalterniflora salt marsh and shellfish sediments areas in northern part of Yellow River Delta by PCR–DGGE

采用PCR-DGGE分子指纹图谱比较黄河三角洲北部互花米草盐沼湿地区与贝壳沉积区的微生物群落结构,对DGGE图谱中10个主条带进行切胶、扩增和测序。结果表明,贝壳沉积区微生物的种类相比较健康的米草区更丰富,贝壳沉积区独有的条带,菌群属于厚壁菌门(Firmicutes)链球菌科(Streptococcaceae)乳球菌属(Lactococcus),与无乳链球菌(S.agalactiae)同源性达100%;以及属于拟杆菌门(Bacteroidetes)黄杆菌目(Flavobacteriales)黄杆菌科(Flavobacteriaceae)的独岛菌属(Dokdonia)和噬纤维素菌属(Cellulophaga),同源性分别为93%与96%。本研究初步揭示了健康的米草区和贝壳沉积区微生物群落多样性特征,为进一步研究海滨盐沼湿地退化机制提供参考。

In this study,PCR DGGE as DNA molecular fingerprinting was applied to analyze the microbial communities in Spartinaalterniflora salt marsh areas and shellfish sediments areas in the northern part of the Yellow River Delta.The DGGE profiles showed the different bands＇patterns in different samples.Ten major bands of 16S rDNA genes fragments from DGGE profiles of sediment samples were further eluted from gel,re-amplified and sequenced.The sequences of these fragments were compared with the database in GeneBank（NCBI）.The results showed that the shellfish sediments areas had more microbial communities compared with healthy Spartinaalterniflora in the salt marsh areas.Unique bands in shellfish sediments areas belong to Lactococcus,sharing 100% homology with S.agalactiae,and Dokdonia as well Cellulophaga,sharing 93% and 96%homology with known sequences.Through the study the primarily microbial communities in Spartinaalterniflora salt marsh areas were found.The shellfish in the sediments areas provided a reference for further research of the degradation mechanism in coastal wetland.