海洋石油降解菌的分离、鉴定和与微藻共培养

Isolation and appraisal of marine petroleum degradation bacteria and co-culture with micro-algae

运用寡营养培养法,从威海金海湾地区的油污中分离出六株石油降解菌,进一步通过16S rDNA序列分析和NCBI数据库的Blast比对分析,对其进行鉴定,编号Y1、Y3和Y7的为假单胞菌属(Pseudomonas),Y2、Y6为芽孢杆菌属(Bacil-lus),Y5为无色杆菌属(Achromobacter)。通过紫外分光光度法测定它们对石油的降解率,培养10 d后,降解条件为30℃、盐度为30、pH8.0、油浓度为60.0 mg/L时,菌株Y6的降解率最大,为81.06%。将菌株与从污水入海口分离得到的微藻共同培养,3 d后菌株Y1、Y5、Y6和Y7的降解率比单独培养时分别增加了3.79%、5.05%、4.42%和7.91%。利用与微藻共生促进细菌降解石油在生物修复方面具有很好的应用潜力。

Sequencing of partial 16S ribosomal DNA（rDNA） genes and intercomparison and analysis of NCBI database was applied to identify six petroleum degrading strains isolated from the Golden Bay in Weihai by extinction culturing technique.It was found that number Y1,Y3 and Y7 were Pseudomonas,Y2 and Y6 were Bacillus,Y5 was Achromobacter.After cultured 10 days under the condition of temperate of 30℃,salinity of 30,pH of 8.0,petroleum concentration of 60.0 mg/L,Y6 showed the biggest petroleum biodegradation rate up to 81.06% measured by ultraviolet spectrophotometry.3 days later,the petroleum biodegradation rate of Y1,Y5,Y6 and Y7 cultured with the micro-algae separated from polluted water in the estuary were increased 3.79%,5.05%,4.42% and 7.91%,respectively than those cultured individually.The accelerating petroleum degradation through symbiosis with the micro-algae has great practical potential for bioremediation.