脂脉宁对酒精性脂肪肝大鼠血清瘦素及肿瘤坏死因子-α含量的影响

Observation of Zhimaining on leptin and TNF-α in rat serum with alcoholic fatty liver

目的观察脂脉宁对酒精性脂肪肝(AFL)大鼠血清瘦素(Leptin)及肿瘤坏死因子-α(TNF-α)含量的影响,探讨脂脉宁治疗AFL的可能作用机制。方法选用Wistar雄性大鼠70只,体质量160～180 g,采用喂饲高脂饲料配合白酒灌胃法复制AFL大鼠模型,随机分为5组,即正常对照组(以下简称正常组),模型对照组(以下简称模型组),脂脉宁高、低剂量组(以下简称高、低剂量组),东宝肝泰对照组(以下简称对照组)。除正常组10只外,其余各组均为15只。正常组用蒸馏水灌胃,进食普通饲料,自由饮水。其余各组造模,同时脂脉宁高、低剂量组分别按0.75、1.5 g/kg体质量灌服脂脉宁混悬液,对照组按0.9 g/kg体质量灌服东宝肝泰混悬液,正常组和模型组灌服蒸馏水,均每日1次,每次用药容积均按1 mL/100 g体质量8,周后,麻醉状态下取股动脉血和肝组织,观察各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、Leptin和TNF-α的含量变化以及肝组织病理形态学变化。结果与模型组比较,脂脉宁高、低剂量组和对照组大鼠血清TC、TG、ALT及AST的含量异常降低(P<0.01);高剂量组大鼠血清TC、TG、ALT及AST的含量低于对照组(P<0.05,P<0.01),低剂量组大鼠血清TC的含量低于对照组(P<0.05)。模型组大鼠血清Leptin、TNF-α的含量均高于其他组(P<0.01);高、低剂量组大鼠血清Leptin、TNF-α含量均低于对照组(P<0.05,P<0.01),而高、低剂量组比较差异无统计学意义(P>0.05)。结论脂脉宁降低血清Leptin和TNF-α的水平可能是其有效防治AFL的作用机制之一。

Objective To observe the effect of Zhimaining on leptin and TNF-α in rat serum with alcoholic fatty liver（AFL）,and discuss possible mechanism of Zhimaining on AFL.Methods 70 Wistar rats（weight from 160~180 g） were randomly divided into five groups,control group,model group,high dose Zhimaining group,low dose Zhimaining group and Dongbaogantai group.AFL model was established by feeding high fat diet and intragastric administration of white spirit.There were 15 rats in each group except 10 rats in control group.Control group was given distilled water,eating normal diet,free access to water.Low dose Zhimaining group and high dose Zhimaining group were respectively fed Zhimaining 0.75,1.5 g/kg body weight.Dongbaogantai group was fed Dongbaogantai 0.9 g/kg body weight.The control group and model group were fed with distilled water.blood and liver tissue was collected under anesthesia.TC,TG,AST,ALT,Leptin and TNF-α in serum and pathological changes in liver tissue were observed after eight weeks.Results TC,TG,ALT and AST in high dose Zhimaining group,low dose Zhimaining group and Dongbaogantai group were significantly decreased in comparison with those in model group（P0.01）.TC,TG,ALT and AST in high dose Zhimaining group were lower than those in Dongbaogantai group（P0.05,P0.01）.TC in low dose Zhimaining group were lower than that in Dongbaogantai group（P0.05）.The levels of leptin and TNF-α in model group were the highest in all groups（P0.01）.The levels of leptin and TNF-α in high dose Zhimaining group and low dose Zhimaining group were lower than those in Dongbaogantai group（P0.05,P0.01）.There was no difference between high dose Zhimaining group and low dose Zhimaining group on the levels of leptin and TNF-α（P0.05）.Conclusion Zhimaining that reduces the levels of Leptin and TNF-α in serum may be one of the mechanism of prevention and treatment of AFL.