解脲脲原体感染对精子功能影响的实验研究

Laboratory study on the function of spermatozoa with infection of Ureaplasma urealyticum

研究解脲脲原体感染对精子功能的影响。正常精液一式二份，一份用解脲脲原体Ⅳ型人工感染正常精子，一份作对照，于感染后４ｈ，８ｈ，１６ｈ，２４ｈ的不同时间观察精子的低渗肿胀试验、精子爬高试验、精子活力及活率。结果，正常精子受解脲脲原体Ⅳ型感染８ｈ，爬高高度低于对照组（４８．１ｍｍ±４．２ｍｍ对６９．５ｍｍ±１．６ｍｍ，Ｐ＜０．０５）；感染１６ｈｇ形精子百分率、活力、活率明显下降；感染２４ｈ精子总肿胀率明显低于对照组（１２．７５％±８．９８％对７５．５０％±４．８８％，Ｐ＜０．０１）。解脲脲原体Ⅳ型对精子低渗肿胀率和毛细管中爬高力有明显影响，是解脲脲原体引起男性不育的病因之一。

Study on the function of spermatozoa which were infected with type Ⅳ of Ureaplasma urealyticum (Uu).Normal semen were divided into two groups,one for factitious infection of Uu,the other for control.The sperm activity and motile ratio,hypoosmotic swelling test(HOS)and sperm penetration in capillary in 4 h,8 h,16 h, and 24 h after infection with Uu.The height of sperm crawling was conspicuously lower in the group which had been infected by Uu for 8 h than in the control group(48 1 mm±4 2 mm in the infected group,69 5 mm±1 6 mm in the control, P <0 05).The ratio of“g”type of sperm,sperm activity and motility ratio descended obviously after Uu infection in 16 hr.The total ratio of HOS was significantly lower in the group of Uu infection in 24 h than that in the control group(12 75%±8 98% against 75 50%±4 88%, P <0 01).The infection of Ⅳ type of Uu affected HOS ratio and activity of sperm penetration in capillary conspicuously and served as one of the reasons which caused male sterility.