藏红花素对大鼠心肌细胞缺氧损伤的保护作用

Protective effects of crocin against hypoxia injury in rat myocardial cells

目的:研究藏红花素(crocin)对缺氧损伤心肌细胞的保护作用,并探讨其作用机制。方法:采用原代培养心肌细胞,建立心肌细胞化学缺氧损伤实验模型。应用生物化学方法测定心肌细胞超氧化物歧化酶(superoxide dis-mutase,SOD)活性和丙二醛(malondialdehyde,MDA)含量;蛋白质印迹法分别检测心肌细胞缺氧诱导因子-1α(hypoxia-inducible factor 1α,HIF-1α)、血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达变化。结果:与缺氧组相比,藏红花素预处理组和阳性对照药组生长状态好,且心肌细胞SOD活性提高(P<0.01),MDA的含量降低(P<0.01)。藏红花素预处理组HIF-1α及VEGF蛋白表达较缺氧组均显著增高(P<0.01)。结论:藏红花素对缺氧损伤心肌细胞具有明显保护作用,作用机制可能与其激活HIF-1α介导的低氧反应通路有关。

Objective： To study the protective effect of crocin and its mechanisms in hypoxia myocardial cells of neonatal rats.Methods： The experimental model of chemical hypoxia was established by primary cultured myocardial cells.The activity of superoxide dismutase（SOD） and the production of malondialdehyde（MDA） in myocardial cells were measured by biochemistry methods.Expression levels of HIF-1α,VEGF protein were detected by western blot.Results： The group pretreated by crocin and positive control group were in a better condition of growth than the group of hypoxic.Moreover,the SOD viability of myocardial cells were increased,while the concentration of MDA was decreased significantly.HIF-1α and VEGF of protein in crocin-retreated myocardial cells was expressed higher than the group of hypoxia.Conclusion： Crocin had better protection in hypoxic injured myocardial cell.The mechanisms of protective effect of crocin may be related to the activation of HIF-1α-mediated pathway of the hypoxia response.