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γ干扰素对肾小球肾炎大鼠肾组织Smad7和Ⅳ型胶原mRNA表达的影响 被引量：2

Effects of IFN-γ on the expression of Smad7 and Col Ⅳ in renal tissue of rat with glomerulonephritis
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摘要 目的:研究IFN-γ对Thy1肾炎模型大鼠肾组织Sm ad7及Ⅳ型胶原表达的影响,从而为阐明IFN-γ抗肾纤维化机制及治疗肾纤维化提供理论和实验依据。方法:制备大鼠抗胸腺细胞血清(antithymocyte serum,ATS)肾炎模型,经尾静脉一次注射IFN-γ15万IU/kg,采用Northern b lot及免疫组化检测病变肾组织Sm ad7、Ⅳ型胶原的表达水平。结果:注射IFN-γ的ATS肾炎模型大鼠肾组织内Sm ad7的表达增加,Ⅳ型胶原mRNA的表达明显减少。结论:IFN-γ可能是通过上调Sm ad7表达而抑制肾组织Ⅳ型胶原表达的机制,对肾小球纤维化起抑制作用。 Objective： To investigate the mechanism of IFN-γ antifibrosis and provide value clues for searching and developing new approaches to the prevention and treatment of organfibrosis,the expression of Smad7 and type Ⅳ collagen was detected in renal tissue of rat ATS glomerulonephritis.Methods： Rat antithymocyte serum glomerulonephritis model was built successfully adopting injection of IFN-γ 15×104 IU/kg vie tail vein once time.Northern blot and immunohistochemistry were employed to identify protein and mRNA expression of Smad7 and type Ⅳ collagen（Col Ⅳ） in renal tissue of rat ATS glomerulonephritis,respectively.Results： IFN-γ could increase the expression of Smad7 and decrease the expression of Col Ⅳ in renal tissue of rat ATS glomerulonephritis.Conclusion： It is possible that IFN-γ can alleviate the development of glomeruli fibrosis by the mechanism of upregulating the expression of Smad7,then downregulating the expression of Col Ⅳ.
作者 姜琳 孙灿林 肖蔚 蒋小芹 李慧 于鸿
机构地区 泰州市人民医院麻醉科 泰州市人民医院病理科 复旦大学上海医学院病理系
出处 《江苏大学学报（医学版）》 CAS 2011年第2期143-146,F0003,共5页 Journal of Jiangsu University：Medicine Edition
基金 上海市科委发展基金重点资助项目(01JC14018) 泰州市311工程人才基金资助项目(201036)
关键词 系膜细胞 Γ干扰素 SMAD7 Ⅳ型胶原 信号转导 肾小球肾炎 mesangial cell interferon-γ Smad7 type Ⅳ collagen signal transduction glomerulonephritis
分类号 R365 [医药卫生—病理学]
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参考文献15

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同被引文献23

  • 1冯凤仪,邓燕,李超雄,李恩华,谈柏浓.扁桃体切除治疗儿童IgA肾病的疗效观察[J].中国医师杂志,2006,8(9):1242-1242. 被引量：7
  • 2杨森,陈双峰,刘伟,耿迎春,孙新中.扁桃体摘除术对IgA肾病患者免疫功能的影响[J].山东医药,2007,47(17):53-54. 被引量：3
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  • 5Lee SM, Rao VM, Franklin WA, et al. IgA Nephropathy: Morphologic Predictors of Progressive Renal Disease [ J ]. Hum Pathol, 1982, 13(4) :314 -322.
  • 6Ebihara I, Hirayama K, Yamamoto S, et al. Th2 predominance at the single-cell level in patients with lgA nephropathy [ J ]. Nephrel Dial Transplant,2001,16 (9) : 1783 - 1789.
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  • 8Ichinose H, Miyazaki M, Koji T, et al. Detection of cytokine mRNA- expressing cells in peripheral blood of patients with lgA nephropathy using non-radioactive in situ hybridization [ J ]. Clin Exp Immunol, 1996,103 ( 1 ) : 125 - 132.
  • 9Suzuki H, Suzuki Y, Aizawa M, et al. Thl polarization in murine IgA nephropathy directed by bone marrow-derived cells [ J]. Kidney Int, 2007,72(3) :319 -327.
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江苏大学学报（医学版）
2011年 第2期

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