Connexin32在胃粘膜肠上皮化生、不典型增生和胃癌中的表达和意义

Expression of connexin 32 in precancerous lesions and carcinoma of the stomach

目的：检测肠上皮化生、不典型增生的胃粘膜组织和胃癌组织中间隙连接蛋白Connexin32（Cx32）的表达，探讨其变化规律及意义。方法：应用免疫组化染色S－P法对21例肠上皮化生、19例不典型增生和32例胃癌标本间隙连接蛋白Cx32表达进行研究。结果：肠上皮化生、不典型增生和胃癌组织中均有不同程度的Cx32表达，但同正常胃粘膜相比，Cx32阳性率明显下降（P＜0．05），在胃癌组织中，Cx32只表达在粘膜下层和肌层的肿瘤细胞内，粘膜层的肿瘤细胞内未见阳性着色。结论：间隙连接可能参与了胃癌发生。

Objective: To investigate the changes of connexin 32 (C×32), a gap junctional protein, in themucosa of intestinal metaplasia, atypical hyperplasia and carcinoma of the stomach and explore its significance. Methods: The expression of C×32 in the mucosa of normal individuals, 21 patients with intestinalmetaplasia, 19 with atypical hyperplasia and 32 with gastric carcinoma was determined with immunohistochemical S-P staining. Results: The positive rate of C×32 expression was significantly lower in patientswith intestinal metaplasia, those with\atypical hyperplasia and those with gastric carcinoma than in the normal individuals (P＜0. 05). In the gastric cancer tissue, the expression of C×32 was seen in the poorlydifferentiated cancer cells that invaded the submucosa and mascularis propria but not the gastric mucosa.Conclusion: The declination of C×32 expression might participate in the carcinogenesis of gastric carcinoma.