TRH与HAD单用及合用抗大鼠高原创伤失血性休克的作用

Effects of TRH or HAD alone and in combination on traumatic hemorrhagic shock at high altitude in rats

目的：在西藏拉萨进行现场实验，观察促甲状腺素释放激素（TRH）与5％NaCl／2．5％醋酸钠／6％右旋糖酐70（HAD）伍用对高原创伤失血性休克大鼠的治疗作用。方法：初进高原大鼠27只，分乳酸林格氏液对照组（8只），TRH治疗组（7只），HAD治疗组（6只）和TRH与HAD合用组（6只），大鼠右侧股骨粉碎性骨折加放血（维持动脉血压6．0hPa1h）复制创伤失血休克模型，观察TRH（5mg／kg），HAD（4ml／kg）和两者合用对创伤失血性休克大鼠血流动力学指标和动物存活时间的影响，以等容量乳酸林格氏液作对照。结果：TRH、HAD单用或合用均能显著提升创伤休克大鼠血压，改善其左室内压（LVSP），左室内压最大变化速率（±dp／dtmax），实测心肌最大收缩速度（Vpm），心肌收缩向量环面积（Lo），明显延长休克动物的存活时间，合用效果稍优于TRH或HAD单用效果。结论：TRH、HAD均具有较好的抗高原创伤失血休克作用，两药可联合用于高原创伤休克的早期救治。

Objective: To investigate the effects of thyrotrophin releasing hormone (TRH) or 5% NaCl/2. 5% acetate sodium/dextran70 (HAD) alone and in combination on traumatic hemorrhagic shock in rats athigh altitude (LaSa, Tibet). Methods: Twenty-seven Wistar rats were employed to establish the model oftraumatic hemorrhagic shock with femeral fracture and bleeding. The animals were given 5 mg/kg of TRH(n= 7) or 6 ml/kg of HAD (n= 6) alone and in combination (n= 6). For the controls (n= 8), equal volumeof lactated Ringer's solution was given. The changes in hemodynamics and survival time of the rats were thenobserved. Results: TRH or HAD alone and in combination could significantly improve the hemodynamic parameters and prolong the survival time of the rats with traumatic hemorrhagic shock. The effects of the tworeagents in combinaion were superior to those of. the two used alone. Conclusion: TRH or HAD alone and incombination can exert significant promoting effects on traumatic hemorrhagic shock at high altitude.