小鼠不同量自体脾组织移植抗大肠杆菌感染的实验研究

Splenic autotransplantation with various amounts for protection of E. coli infection in mice

目的：为了研究自体脾组织移植抗大肠杆菌的功能及确定理想的移植量。方法：不同量自体脾组织移植半年后，用大肠肝菌及125I标记的大肠杆菌从腹腔和血中感染小鼠，用图像分析法观察原脾及移植牌中脾小体数量和面积。结果：脾切除动物感染大肠杆菌后，存活率较假手术组明显降低；脾切除后，肺脏清除大肠杆菌的能力显著降低并引起血中大肠杆菌潴留；小鼠移植再生脾的形态结构及脾小体数量和面积和原脾非常接近，但当移植量达到原脾重量的2／3时，也可出现再生不够完善的现象。结论：发现1／2原脾重量的移植量是小鼠自体脾组织移植抗大肠杆菌的理想移植量，脾切除可以影响肺脏清除大肠杆菌的功能，自体脾组织移植可以恢复其功能。

Objective: To investigate the role of splenic autotransplantation in protection of E. coli infection and determine the proper transplanted amount. Methods: Splenic autotransplantation with variousamounts was conducted in mice. Six months later, E. coli and 125I-labeled E. coli were injected into the animals. The number and area of splenic nodules were determined with image analysis. Results: Splenectomydecreased the survival rate after E. coli challenge and influenced the function of lung and blood to clear E.coli. Meanwhile, the structure, number and area of splenic nodules in the regenerated spleen were similar tothose in the original spleen. Conclusion: The proper transplanted amount of the spleen is half of the weightof the original spleen. The pulmonary function of clearing E. coli is decreased after splenectomy. The splenicautotransplantation can resume this function.