人参皂甙Rg1、Rb1和维生素E对氧化损伤的牛RPE细胞凋亡的影响

Influence of ginsenoside Rg1,Rb1 and vitamin E on apoptosis of oxidized bovine RPE cells in vitro

目的检验氧化损伤的牛ＲＰＥ细胞是否存在着凋亡以及人参皂甙Ｒｇ１、Ｒｂ１和维生素Ｅ对其的影响，以期进一步研究ＲＰＥ细胞氧化损害的机理，并试图为预防和治疗与ＲＰＥ细胞相关的视网膜疾病提供有效的手段。方法建立牛ＲＰＥ细胞氧化损伤模型，利用原位末端标记技术测定ＲＰＥ细胞凋亡。结果发生凋亡的ＲＰＥ细胞细胞核染成紫蓝色，形态不一，往往成颗粒状着色，与次黄嘌呤（ＨＸ）／黄嘌呤氧化酶（ＸＯ）（ＨＸ＝０．１ｍｍｏｌ·Ｌ－１，ＸＯ＝５Ｕ·Ｌ－１）组阳性细胞数相比较，正常对照组Ｒｇ１（０．１ｍｇ·Ｌ－１）组及维生素Ｅ（１０ｍｇ·Ｌ－１）组存在显著性差异（Ｐ＜０．０５），而与Ｒｂ１（１０ｍｇ·Ｌ－１）组无显著性差异。结论氧化损伤可使培养的牛ＲＰＥ细胞发生凋亡。人参皂甙Ｒｇ１和维生素Ｅ对抗氧自由基对ＲＰＥ细胞损伤的机制可能是通过清除氧自由基、减少ＲＰＥ细胞脂类过氧化的发生，从而有效地抑制ＲＰＥ细胞凋亡，利于ＲＰＥ细胞的生存。

Objective To investigate whether apoptosis is exited in bovine RPE cells damaged by oxygen free radicals,and whether ginsenoside Rg1Rb1 and vitamin E have an influence on apoptosis of oxidized RPE cells.]Methods The experimental model of oxidized bovine RPE cells was made.In these cells,apoptosis was evaluated by in situ TdTmediated biotindUTP nickend labeling(TUNEL). Results Apoptosis was visualized by the in situ endlabeling in RPE cells,the apoptosis cells' nucleus were stained purp lish blue.Compared to the control,the number of apoptosis cells with hypoxanthine(HX)/xanthine oxidase(XO) (HX=0.1mmolL-1, XO=5UL-1) treatment significantly increased (P<0.05).Rg1(0.1mgL-1)),and vitamin E (10mgL-1)significantly decreased the number of apoptosis RPE cells in oxidized RPE cells in comparision with HX/XO treatment(P<0.05),Rb1(10mgL-1)had no significant effect(P>0.05). ConclusionsTBZOxygen free radicals can result in apoptosis.Ginsenoside Rg1 and vitamin E can inhibit the apoptosis,Rb1 had no effect on it.These results indicate that Rg1 and vitamin E probably inhibited apoptosis by clearing free radicals and decreasing lipid peroxidation of bovine RPE cells.