雌激素促进人的类成骨细胞TE85成骨作用的受体机制

RECEPTOR MECHANISM OF ESTROGEN STIMULATING BONE FORMATION IN HUMAN OSTEOBLAST LIKE CELL LINE TE85

目的：研究雌激素促进人的类成骨细胞株ＴＥ８５细胞骨形成的作用。方法：用３Ｈ胸腺嘧啶、３Ｈ脯氨酸参入法分别测定细胞的增殖和胶原合成；紫外分光光度法测定细胞内骨碱性磷酸酶（ＡＬＰ）活性；放免法测定细胞内骨钙素（ＢＧＰ）含量；放射配基法测定细胞核雌激素受体结合。结果：雌激素（Ｅ２，００１～１０ｎｍｏｌ·Ｌ－１）可浓度依赖地刺激ＴＥ８５细胞的３Ｈ胸腺嘧啶和３Ｈ脯氨酸的参入量；在相同的时间点和剂量下，可增加成骨细胞内ＡＬＰ活性和ＢＧＰ的含量。结论：Ｅ２通过增加成骨细胞数量、促进细胞胶原蛋白及ＡＬＰ和ＢＧＰ的合成而促进成骨作用，这些作用是通过雌激素受体介导的。

AIM: To study the effects of estrogen on stimulating bone formation in osteoblast like cell line TE85. METHODS: Utilizing methods of 3H thymidine incorporation for cell proliferation, 3H proline incorporation for collagen synthesis, ultraviolet spectrophotometry for measurement of alkaline phosphatase (ALP) activity, radioimmunoassay for bone gla protein (BGP) content assay, and radio ligand binding for estrogen receptor assay. RESULTS: Cells were incubated with various concentrations (from 0 01 to 10 nmol·L -1 ) of 17β estradiol (E 2) for 48 h and 72 h, 3H thymidine incorporation into DNA was increased gradually while 3H proline incorporation was enhanced in the same fashion. E 2 at the same dose increased the intracellular ALP activity and BGP content at the same time. In ligand receptor binding experiment, the K D of 3H E 2 saturation test was 2 59×10 -10 mol·L -1 in TE85 cells and the receptor number was found to be 262 7±64 6 site per cell. The IC 50 of 4 OH tamoxifen and ICI 182,780 were 0 99×10 -8 mol·L -1 and 8×10 -10 mol·L -1 , respectively. CONCLUSION: E 2 improved bone formation by ways of stimulating cell proliferation, enhancing collagen synthesis, ALP activity, and BGP content. The effects of E 2 were performed by the mediation of estrogen receptor.