油菜菌核病生防芽孢杆菌的分离鉴定及其脂肽化合物分析

Screening of Bacillus strains with high inhibition on rape Sclerotinia disease and its lipopeptide compounds detection

采用平板拮抗筛选,分别从西藏日喀则地区和拉萨地区杂草根围土壤中筛选到2个对油菜菌核病菌有显著拮抗活性的芽孢杆菌菌株RJGP16和YBWC43。通过生理生化鉴定、16S rDNA序列分析和BOX-PCR指纹图谱分析,鉴定菌株RJGP16为萎缩芽孢杆菌Bacillus atrophaeus,菌株YBWC43为解淀粉芽孢杆菌Bacillus amyloliquefaciens。离体叶片试验结果显示,菌株RJGP16和YBWC43对油菜菌核病菌防治效果分别为50.24%和100.00%。脂肽化合物种类分析显示,菌株RJGP16产生脂肽化合物表面活性素和芬枯草菌素,菌株YBWC43产生杆菌霉素D和芬枯草菌素。表明菌株RJGP16和YBWC43对油菜菌核病的防治效果与其产生的脂肽化合物有关。

In order to screen Bacillus spp.with high antagonistic activity towards Sclerotinia sclerotiorum,the causal organism of oilseed rape Sclerotinia disease,the dual culture method was used for screening of high biocontrol activity Bacillus strains.Two strains RJGP16 and YBWC43 were isolated from rhizospheres of grass which collected from Xigaze and Lhasa in Tibet.According to the biochemical tests,physiological tests,16S rDNA sequences analysis and BOX-PCR fingerprints analysis,RJGP16 and YBWC43 were identified as B.atrophaeus and B.amyloliquefaciens respectively.Biocontrol efficacy of RJGP16 or YBWC43 on oilseed rape Sclerotinia disease in the detached leaves was 50.24% or 100.00%,respectively.The lipopeptide compounds analysis showed that strain RJGP16 produced surfactin and fengycin,while strain YBWC43 produced bacillomycin D and fengycin,indicating that the biocontrol efficacy of these two strains towards S.sclerotiorum were related to the antifungal lipopeptide compounds produced by strains themselves.