铜绿假单胞菌ZB27的定殖能力及对杂交竹梢枯病的防控作用

Colonization of Pseudomonas aeruginosa ZB27 and its control effect on hybrid bamboo blight

通过抗生素标记法获得铜绿假单胞菌ZB27的抗氯霉素突变菌株,并运用喷雾和稀释平板法检测该菌的定殖能力及对杂交竹梢枯病的防控作用。结果表明,ZB27标记菌株抗氯霉素的浓度为750μg/mL,且性状稳定、回收率高。ZB27可以快速定殖到杂交竹枝叶,并长期维持稳定的定殖量;高浓度(109、107CFU/mL)的菌液定殖量大于低浓度的菌液;在叶上的定殖量大于枝干,8天时达到顶峰,到40天时仍能保持较高的水平;病原菌的接入对生防菌在叶部的定殖影响较大,对在枝干定殖影响较小。预先接种ZB27菌悬液和其次生代谢产物对杂交竹梢枯病的防效分别为70.07%和68.05%,显著高于其它处理,说明利用菌悬液或其次生代谢物防治杂交竹梢枯病均有较好的防治效果。

A mutant strain of Pseudomonas aeruginosa ZB27 resistant to chloramphenicol was obtained using the antibiotic label method.The colonization ability and control effect were determined with the spray and diluting plate methods.The result indicated that the concentration of labeled strain ZB27 resistant to chloramphenicol was 750 μg/mL with stable characterization and high recovery.The labeled strain ZB27 could colonize rapidly on the leaves and branches and maintain steady colonization amount in a considerably long time.The number of colonization with high-concentration（109,107 CFU/mL） were higher than that with the low-concentration.The colonization on the leaves was higher and had longer alive period,which reached the peak at 8th d and kept high level till 40th d.The colonization of pathogen to biocontrol bacteria had great effects on leaves but little on branches.The control effect of inoculation bacterial suspensions or secondary metabolites was significant higher than the other treatments with 70.07% or 68.05%,respectively,indicating that bacterial suspensions and secondary metabolites had good effects for controlling hybrid bamboo blight.