2,4-D胁迫下土壤酶活性对外源物质的响应

The Responses of Soil Enzyme Activities to Exogenous Substances under 2,4-D Stress

采用三因素完全随机设计,通过盆栽试验,研究2,4-D胁迫下添加多效唑、尿素对土壤脲酶、碱性磷酸酶、蛋白酶活性的动态影响。结果表明,不同质量分数2,4-D对土壤脲酶、碱性磷酸酶和蛋白酶活性的影响不同;1～60 d,与对照相比,低质量分数2,4-D对土壤脲酶、碱性磷酸酶活性表现为激活效应,而对蛋白酶活性表现为抑制作用;高质量分数2,4-D则抑制了土壤脲酶、蛋白酶活性,而对碱性磷酸酶活性表现为激活效应;添加多效唑和尿素对土壤脲酶、碱性磷酸酶和蛋白酶活性均表现为激活效应,特别是对脲酶和蛋白酶活性的平均激活率最高可达44.88%和63.18%。

This experiment was carried out through pot culture test with three-factor completely randomized design.The dynamic effect of paclobutrazol and urea on soil urease,alkaline phosphatase and protease activities under 2,4-D stress were studied.The results showed that the influences of 2,4-D on soil urease,alkaline phosphatase,and protease activities were different.Compared with the control,low concentration 2,4-D activated the soil urease and alkaline phosphatase activities,but suppressed protease activity during 1～60 days.High concentration 2,4-D inhibited the soil urease and protease activities,but activated alkaline phosphatase activity.Paclobutrazol and urea had activation effect on soil urease,alkaline phosphatase and protease activities,especially on soil urease and protease activities.The average activation rate was up to 44.88% and 63.18% respectively.