加味丹参饮对心肌缺血再灌注损伤血瘀证兔肿瘤坏死因子α和白细胞介素-2的影响

Influence of Jiawei Danshen Decoction on TNF-α and IL-2 of Rabbits with Myocardial Ischemia Reperfusion and Blood Stasis Syndrome

目的研究加味丹参饮对心肌缺血再灌注损伤(IRI)血瘀证兔肿瘤坏死因子-α(TNF-α)和白细胞介素-2(IL-2)的影响,探讨对心肌IRI的保护作用机制。方法家兔60只,随机分为A组(空白组)、B组(血瘀证组)、C组(IRI组)、D组(预处理组)、E组(丹参组)、F组(加味丹参饮组),每组10只。建立血瘀证兔IRI模型,采用加味丹参饮对该模型进行干预,观察炎症因子TNF-α和IL-2以及乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB)的水平变化。结果 IRI组TNF-αI、L-2、LDH和CK-MB水平明显升高,与空白组比较有统计学意义(P<0.05);加味丹参饮可以降低TNF-αI、L-2、LDH和CK-MB水平,与IRI组比较亦有统计学意义(P<0.05)。结论加味丹参饮可以降低炎症因子水平,防止心肌细胞损伤,这是其保护心肌IRI作用机制之一。

Objective To study the influence of Jiawei Danshen decoction（JDD） on tumor necrosis factor alpha（TNF-α） and interleukin-2（IL-2） of rabbits with myocardial ischemia reperfusion injury（IRI） and blood stasis syndrome.Methods Sixty rabbits were randomly divided into 6 groups（10 rabbits in each group）： Control group（A）,blood stasis syndrome group（B）,IRI group（C）,precondition group（D）,Danshen group（E）,and JDD group（F）.The models with IRI and blood stasis syndrome were established.The levels of serum TNF-α,IL-2,lactate dehydrogenase（LDH） and creatine kinase-MB（CK-MB） were detected by radio immune assay.Results Compared with control group,the levels of serum TNF-α,IL-2,LDH and CK-MB were increased in IRI group（P0.05）.Compared with IRI group,the levels of serum TNF-α,IL-2,LDH and CK-MB were decrease in JDD group（P0.05）.Conclusion JDD might play an important role in the modulation of inflammatory factors in myocardial IRI by preventing of myocardial IRI.