深圳汉族Kir6.2基因与2型糖尿病关系的病例-对照研究

Correlation of Membrane Glycoprotein Kir6.2 Gene Polymorphism with Type 2 Diabetes Mellitus and Insulin Resistance in Han Nationality in Shenzhen

目的研究深圳汉族人群钾离子内向整流通道Kir6.2基因多态性与胰岛素抵抗(IR)特征和2型糖尿病(T2DM)的关系。方法运用聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)技术对深圳市251例T2DM患者和170例非DM对照(NGT)人群的Kir6.2基因第一外显子E23K多态性进行分析,并对T2DM组不同基因型间临床及生化指标进行比较。结果 T2DM组与非DM对照组比较,Kir6.2基因型频率和等位基因频率分布差别均无统计学意义;T2DM组携带K等位基因者空腹血糖(FPG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及空腹血C肽(CP)水平显著高于携带E等位基因者(P<0.05)。结论深圳市汉族人群Kir6.2基因E23K多态性与2型DM患者胰岛素抵抗表型相关,但与T2DM的发生无明显关联。

Objective To explore the relationship of the E23K polymorphism of membrane glycoprotein Kit6.2 gene with the features of insulin resistance （IR） and type 2 diabetes mellitus （T2DM） in Han nationality of Shenzhen. Methods The E23K polymorphism in exon 1 of Kit6.2 gene were determined with the technique of polymerase chain reaction-denaturing gradient gel electrophoresis （PCR- DGGE） in 251 type 2 diabetic subjects and 170 normal glucose tolerance （NGT） control subjects in Shenzhen city. The clinical and laboratory data were also compared between type 2 diabetic patients with different genotypes. Results No statistically significant differences were observed in the genotype and allele frequencies between control subjects and type 2 diabetic subjects. The K allele was associated with higher fasting plasma glucose （FPG）, total cholesterol （TC）, low density lipoprotein cholesterol （LDL-C） and higher C-peptide （CP） concentrations than the E allele was in type 2 diabetic patients （P〈0.05）. Conclusion The present study shows that the E23K polymorphism of Kir6.2 gene in Han nationality of Shenzhen is not associated with the occurrence of type 2 diabetes meihtas, but associated with insulin resistance in type 2 diabetic patients.