石榴汁和苹果汁干预对老龄大鼠肝、心、脑组织自由基代谢的影响

Effect of Pomegranate Juice and Apple Juice on Free Radical Metabolism in Liver,Heart and Brain of Aged Rats

目的研究抗氧化活性较强的石榴汁和较弱的苹果汁对老龄大鼠肝、心、脑组织自由基代谢的影响。方法自然衰老Wistar大鼠(22月龄)30只,随机分为三组,分别灌胃石榴汁3.2 ml,苹果汁2.4 ml和蒸馏水3.2 m(l对照组),实验周期4周,实验结束后取大鼠肝、心、脑组织制作匀浆液测定总抗氧化力、丙二醛(MDA)及自由基含量。结果石榴汁干预组老龄大鼠肝脏、脑组织抗氧化能力显著升高、自由基水平明显下降,肝、心、脑MDA含量均显著降低,与对照组比较差异有统计学意义;苹果汁干预后老龄大鼠肝组织MDA含量及自由基水平明显降低。苹果汁、石榴汁对老龄大鼠心脏抗氧化能力及自由基水平均无显著性影响。结论抗氧化活性较高的石榴汁改善衰老大鼠肝、心、脑组织自由基代谢能力较强,而抗氧化活性较低的苹果汁作用稍弱。

Objective To study the effect of pomegranate juice and apple juice on free radical metabolism in the liver, heart and brain tissues of aged rats. Methods Thirty aged Wistar rats （22 months old） were randomly divided into three groups： pomegranate juice and apple juice as two experimental groups, while distilled water as normal control group. They were administrated fruit juices or distilled water respectively by garage daily for 4 weeks. At the end of experiment, total antioxidant capacity （TAOC）, content of MDA and free radical of the liver, heart and brain tissues were measured. Results The aged rats in pomegranate group showed significantly higher TAOC and lower content of free radicals in liver and brain respectively, while the concentrations of MDA in the liver, brain and heart tissues declined remarkably as compared to control group. The concentration of MDA and content of free radical in the liver tissue of aged rats in apple juice group decreased significantly. The pomegranate juice and apple juice had no evident effect on TAOC and concentration of free radical in the heart tissue of aged rats. Conclusion The pomegranate juice which showed stronger antioxidant capacity in vitro possessed higher capability to improve the metabolism of free radical in the liver, heart and brain tissues of aged rats.