枇杷叶中齐墩果酸的提取工艺

Study on Extraction of Oleanolic Acid from Eriobotrya Japonica

以微波法进行提取,采用最优的薄层分离-比色显色法测定齐墩果酸含量,确定出微波提取的最佳工艺为:乙醇浓度95%、提取功率为630 W、微波时间为70 s、料液比为1∶20(g/mL)。微波法与传统的热回流法、冷浸取法进行比较,得出微波法的提取率为84.8%,热回流法的提取率为64.1%,冷浸法的提取率为61.3%,即微波法提取率明显高于其他2种方法,且结果显示微波法提取齐墩果酸有效可行。

Microwave extraction method,using the best thin-layer separation-Colorimetric Determination of Color oleanolic acid content,identify the optimum microwave extraction as follows： 95 % ethanol concentration,extraction power of 630 W,microwave time 70 s,solid-liquid ratio of 1∶20.Microwave method and conventional thermal reflux method,the cold soak comparison emulated obtained by microwave method of extraction rate of 84.8 %,heat reflux extraction rate was 64.1 %,the cold soak method of extraction rate was 61.3 %,or microwave extraction was significantly higher than the other two methods,and the results showed that microwave extraction of oleanolic acid to be effective.