摘要
建立饮料中胭脂树橙色素的高效液相色谱测定法。饮料样品经过适当的稀释后,调节pH为8-8.5,直接进样用HPLC测定样品中胭脂树橙色素的含量。色谱柱为ZORBAX Eclipse XDB-C18（5μm,4.6 mm i.d.×250 mm）,以V（乙腈∶VC 1%的乙酸溶液）=75∶25（体积比）为流动相,紫外检测器测定;回收率为90.0%-107.4%,相对标准偏差小于2.95%,定量限（S/N≥10）为0.25 mg/kg。
The method for the determination of annatto extract in beverage was studied.After diluted and adjust pH to 8-8.5,the norbixin content was analysed by LC on a column of ZORBAX Eclipse XDB-C18（5 μm,4.6 mm i.d.×250 mm） with V（acetonitrile）∶V（1 % acetic acid）=75∶25 as mobile phase and detected at 460 nm with VWD;limit of quantification（LOQ） was 0.25 mg/kg and the recovery 90.0 %-107.4 %,the relative standard deviation was less than2.95 %.
出处
《食品研究与开发》
CAS
北大核心
2011年第4期121-123,共3页
Food Research and Development
关键词
胭脂树橙
饮料
高效液相色谱
annatto extract
beverage
high performance liquid chromatography
