摘要
建立以原花青素B2为标准品测定葡萄籽提取物中原花青素含量的方法。以含量≥99%的原花青素B2(HPLC标定)做标准品,参照Bate-Smith法,在546 nm处测定吸光度,来标定葡萄籽提取物中原花青素含量。原花青素B2通过Bate-Smith法反应后的吸光度与浓度成良好线性关系,其线性范围为22.89μg/mL～114.5μg/mLr,=0.999 02(n=5)。回收率:97.99%,RSD∶1.02%(n=6)。该法以通过HPLC标定的原花青素单一成分B2做为标准品,解决了过去以原花青素混合物做标准品其原花青素含量标准不统一,无法让人信服的问题,其测定结果能准确反映葡萄籽提取物中的原花青素的真实含量,可用作质控方法。
To establish a method for detecting proanthocyanidins in grape seed extract with proanthocyanidin B2 standard.Refer to the Bate-Smith method,using proanthocyanidin B2（≥99;HPLC） to calibrate proanthocyanidins in grape seed extract at 546 nm.Linear range of proanthocyanidins is 22.89 μg/mL-114.5 μg/mL（r=0.999 02,n=5）,Recovery∶97.99 %,RSD∶1.02 %（n=6）.Using single ingredient proanthocyanidin B2 standard calibrated by HPLC to calibrate proanthocyanidins,resolving the problem that the standard content is not identical and can not convince when using proanthocyanidin compound standard.The method have better linearity and repeatability,can detect grape seed extract＇s proanthocyanidins exactly.
出处
《食品研究与开发》
CAS
北大核心
2011年第4期141-142,共2页
Food Research and Development
