成团泛菌低分子脂多糖的急性毒性、刺激性、致敏性及遗传毒性评估

Evaluation of the Acute Toxicity,Irritation,Hypersensitivity and Genetic Toxicity for Lipopolysaccharides from Pantoea agglomerans (LPSp)

本研究对成团泛菌低分子脂多糖(Pantoea agglomerans lipopolysaccharide,LPSp)的安全性进行初步评估。本研究采用一次限量法,用昆明种小鼠进行LPSp急性经口毒性试验,了解LPSp的急性毒性;采用新西兰兔分别进行LPSp急性和多次皮肤刺激性试验以及急性眼刺激性试验,了解LPSp的皮肤和粘膜刺激性;采用豚鼠进行LPSp皮肤变态反应试验,了解LPSp的致敏性;应用平板掺入法进行鼠伤寒沙门氏菌/回复突变试验和小鼠骨髓细胞微核试验考察LPSp的遗传危害。急性毒性试验结果显示,LPSp对小鼠经口一次灌胃的LD50大于5000mg/kg体重,属实际无毒级别;LPSp急性和多次皮肤刺激性试验以及急性眼刺激性试验结果显示,皮肤刺激和眼刺激积分均为0分,LPSp对皮肤无刺激性、对眼睛无急性刺激性;在皮肤变态反应试验中,LPSp在各观察时间点的皮肤变态反应积分均为0分,其致敏率均为0%,说明LPSp对豚鼠无致敏性;LPSp的鼠伤寒沙门氏菌/回复突变试验结果呈阴性(P>0.05);LPSp的小鼠骨髓细胞微核试验结果亦呈阴性,LPSp各剂量组的微核发生率与阴性对照组未见统计学差异(P>0.05),而与阳性对照组有明显差异(P<0.01)。本研究结果表明,在本实验剂量范围内,LPSp对小鼠经口毒性极低,属实际无毒级别,对家兔皮肤和眼睛无明显刺激性,对豚鼠无致敏性,对所试菌株和小鼠体细胞无诱变性和致突变性。

In this study,a serial of experiments were practiced to evaluate the safe of Pantoea agglomerans lipopolysaccharide （LPSp）.Using limited does method,the acute oral toxicity test was carried out on Kunming mice to understand acute toxicity of LPSp;the skin and eye Irritation test were carried out on New Zealand Rabbits to understand the skin and eye irritation of LPSp;and the skin allergy test were carried out on guinea pig to understand the hypersensitivity of LPSp.The salmonella typhimurium/reverse mutation assay and cell micronucleus test was conducted to understand the genetic toxicity of LPSp.The result of acute oral toxicity test showed that LPSp is defined with no toxicity （LD505 000mg/kg）;which of the skin and eye irritation test showed LPSp hasn＇t irritation reaction and hypersensitivity for skin and eye （the integration of both skin and eye are 0）;which of skin alleray test showed LPSp is not the sensitinogen for guinea pig （both integration and ratio of skin reaction are 0）;which of sal-monella typhimurium/reverse mutation assay was negative （P0.05）;which of cell micronucleus test was negative （compared with negative control P0.05,compared with positive control P0.01）.It is concluded that LPSp is very safe for these animals in our experiments;it is no toxicity,no irritation,no hypersensitivity and no genetic toxicity.