地锦草体外抑菌有效部位的筛选试验

Selection of bacteriostasis positions of Euphorbia humifusa wild in vitro

采用水煎、水醇和五部位提取法从地锦草中提得组成成分不同的７ 种提取物，以肠道致病性大肠杆菌Ｏ７８∶Ｋ８０，沙门氏菌Ｃ７９－１，金黄色葡萄球菌为目的菌株进行体外抑菌试验。结果表明，水煎提取物对大肠杆菌Ｏ７８∶Ｋ８０，沙门氏菌Ｃ７９－１，金黄色葡萄球菌的最小抑菌质量浓度分别为１０，１０，１２．５ ｇ／Ｌ；水醇提取物对上述试验菌的最小抑菌质量浓度依次为５，７．５，１０ ｇ／Ｌ；五部位法所得５ 种提取物的抑菌浓度均大于１５

Seven kinds of extract were obtained from Euphorbia humifusa Wild by water decoction,water ethanol and five position methods,and were conducted bacteriostasis test with intestine pathogenic Colon bacillus O 78 ∶K 80 ,Salmonella Pullorum C 79 1 ,staphylococcus aureus for target bacteria in vitro .The results indicated that the minimum concentration of bacteriostasis of water decoction extract for Colon bacillus O 78 ∶K 80 ,Salmonella Pullorum C 79 1 staphylococcus aureus was 10,10,12.5 g/L crude drug,respectively.The minimum concentration of bacteriostasis of water ethanol extract on abive nebtuibed test bacteria was 5,7.5,10 g/L crude drug,successively.The concentration of bacteriostasis of five kinds of extract which obtained by five position method was higher than 15 g/L crude drug.