葡萄NAC转录因子家族生物信息学分析

Bioinformatics Analysis of the NAC Transcription Factor Family in Grapevine

NAC基因家族是植物所特有的一类重要转录因子,广泛参与植物生长、发育以及器官建成、逆境胁迫应答等反应。目前,基因组信息的挖掘和分析已经成为葡萄功能基因组学研究的重要组成部分。本文利用生物信息学方法对葡萄143条NAC蛋白序列的系统发生和NAC基因组定位进行分析,然后对其氨基酸组成成分、理化性质以及二级和三级结构进行预测和分析,同时还分析了葡萄与拟南芥的NAC基因家族之间的联系。结果显示这143条蛋白序列与拟南芥94个NAC基因蛋白序列一起分成了16个亚族,说明拟南芥与葡萄NAC基因间具有较高的保守性。基因组定位结果发现143条NAC基因分布在17条染色体上。研究还发现不同亚家族间氨基酸数目、氨基酸序列间的疏水性存在一定的差异;而它们的二级结构都主要以随机卷曲为组成部分,且143条氨基酸序列三维结构十分相似。本文试验结果将为葡萄NAC基因家族的进一步功能分析提供重要研究基础。

NAC gene family is a specific kind of important transcriptional factors to plants,which are involved extensively in plant growth,development and organogenesis and stress responses.Now,grape has been focused on molecular biology research areas,of which the informationmining and analysis of grape genome is very significant for the progress of grape functional genomics.In this article,by utilizing the bioinformatics method,we analyzed the phylogenetic of 143 NAC family protein sequence and NAC genome localization firstly,and then predicted and analyzed their amino acid composition,physical and chemical characteristics,as well as secondary and tertiary structures,meanwhile,we still assayed the relation of NAC gene family between grapevine and Arabidopsis thaliana.The result showed that these 143 amino acid sequences could be clustered into sixty groups together with 94 NAC sequences of Arabidopsis thaliana,which illuminated that NAC genes had highly conservative between grapevine and Arabidopsis thaliana.Further genetic mapping of NAC genome localization found that they could be distributed on 17 chromosomes.The research still found that the number of amino acid and hydrophobic of amino acid sequences in different subfamilies presented some differences,however,the results of secondary structure predicted that the main composition of 143 amino acid sequences was random curl and the tertiary structure of all the sequences were quite similar.The results of this investigation could definitely provide a significant foundation for further research on the function analysis of grapevine NAC gene family.