‘黑农51’子叶节和胚尖再生体系的建立及优化

Stablishment and Improvment on Regeneration Systems of'Heinong51'Cotyledonary Nodes and Embryonic Tips

为建立一个高效的大豆再生体系用于大豆的遗传转化,选用‘黑农51’的子叶节和胚尖作为外植体,建立了黑农51的子叶节和胚尖再生体系,并研究了6-BA和IBA对大豆再生的影响。结果表明,‘黑农51’子叶节最适芽诱导培养基为MSB5+1.0mg/L6-BA+0.2mg/LIBA,最适生根培养基为MSB5+1.0mg/LIBA;胚尖最适芽诱导培养基为MSB5+0.4mg/L6-BA+0.2mg/LIBA,最适生根培养基为MSB5+2.0mg/LIBA。‘黑农51’子叶节再生体系的出芽率、出芽数、芽伸长数和生根率4个指标均高于胚尖再生体系,更适合作为遗传转化的受体材料。

To establish a high frequency regeneration system for the gene transformation of soybean（Glycine max（L.）） Merr.cotyledonary nodes and embryonic tips from ＇ Heinong51 ＇ were used as the explants.Regeneration systems of cotyledon nodes and embryonic tips from ＇ Heinong51 ＇ were established in this research.Effect of 6-benzylaminopurine（6-BA） and indolebutyric acid（IBA） on soybean regeneration was studied.The results indicated that the optimal shooting induction medium of cotyledonary node from ＇ Heinong51 ＇ was MSB 5 added 1.0 mg/L 6-BA and 0.2 mg/L IBA and the optimal rooting medium of cotyledonary node was MSB 5 added and 1.0 mg/L IBA.The optimal shooting induction medium of embryonic tip from ＇ Heinong51 ＇ was MSB 5 added 0.4 mg/L 6-BA and 0.2 mg/L IBA and the optimal rooting medium of embryonic tip was MSB 5 added and 2.0 mg/L IBA.In comparison with regeneration system of embryonic tips,＇ Heinong51 ＇ regeneration system of cotyledon nodes was superior in shoot regeneration frequency,shoot regeneration number and shoot elongation number.The cotyledon nodes from ＇ Heinong51 ＇ was more suitable for receptor materials in soybeans genetical transformation.