烟蚜茧蜂畸形细胞对寄主生理代谢的调控

Regulation of Aphidius gifuensis teratocytes to physiological Metabolism of Aphis

研究畸形细胞对寄主蚜虫的生理生化影响及其自身发展变化。分别采用Folin-酚法、SDS聚丙烯酰胺凝胶电泳法、薄层层析法和索氏抽提法测定寄生和未寄生寄主血淋巴的蛋白质含量、蛋白质组成、糖类含量和脂肪含量变化。寄生寄主与未寄生寄主相比,生理代谢受到抑制。血淋巴蛋白和甘油酯含量随日龄逐渐增加到第3天达最大后下降,而糖含量逐渐降低,分析是畸形细胞分泌和合成的结果。同时,经SDS-PAGE电泳显示,血淋巴中新出现15.9kDa和51.1kDa两种特异的蛋白质。通过对寄主血淋巴主要成分的分析,烟蚜茧蜂畸形细胞对寄主的发育起着重要的调控作用。

Investigate effects of Aphidius gifuensis teratocytes on physiological metabolism of aphis and change themself.In the study,folin-phenol,SDS-PAGE,thin layer chromatography and soxhlet extractor method were used to determine the content of protein,protein composition,the quantity of sugar and glyceride for haemolymphe in parasitized and non-parasitized host.It was indicated that the metabolism of parasitized aphid was constantly inhibited as compared with that of the non-parasitized host.The level of protein and glyceride was increasing with instars to maximum in the third day,then lower,but suger content declined gradually,may be due to secretion and synthesis of teratocytes.At the same time,SDS-PAGE analysis showed that 15.9kDa and 51.1kDa parasitic specific protein was appeared in the hemolymph.Through the analysis of main components for haemolymphe in parasitized and non-parasitized host,Aphidius gifuensis teratocytes plays an important regulatory role in the development of the host.