结核分枝杆菌eis基因突变与氨基糖苷耐药的研究

Relationship Between Enhanced Invracellular Survival Gene Mutation in Mycobacterium Tuberculosis and Aminoglycoside-resistance

目的:探讨结核分枝杆菌eis基因突变与氨基糖苷耐药之间的相互关系。方法:以本室保存的35株已确定耐一线药物(异烟肼、利福平、乙胺丁醇、链霉素)的结核分支杆菌为研究对象,应用BECTEC960测定其二线药物(阿米卡星、卡那霉素)的耐药情况,同时应用基因测序的方法测定结核分枝杆菌eis基因突变情况,分析eis基因突变与氨基糖苷耐药之间的相互关系。结果:氨基糖苷耐药的部分结核分杆杆菌中,eis基因487位碱基出现突变,相应的163位氨基酸密码子由CGT突变为CAT,即由缬氨酸变为异亮氨酸。结论:eis基因V163I突变(缬氨酸变为异亮氨酸)可能与结核分枝杆菌耐氨基糖苷类药物有关。

Objective：To investigate the relationship between enhanced invracellular survival（eis） gene mutations and aminogly-coside-resistance in MTB.Methods：35 strains of MTB resistant to isoniazid,rifampicin,ethambutol and streptomycin were chosen,the resistance to amikacin and kanamycin were determined by BECTEC 960,eis gene mutations were identified by PCR and sequencing.Results：There was Eis gene mutation of G487A in 4 strains and the amino acid changed from valine to isoleucine.Conclusion：The eis V163I mutation might associate with aminoglycoside-resistance in MTB.