氢氧化钠瘤内注射通过抗血管生成作用抑制肝癌生长的机制研究

Effect of Intratumoral Injection of Sodium Hydroxide in Inhibition of Liver Tumor Growth

目的:研究氢氧化钠溶液瘤内注射对肝癌的生长抑制作用并探索其机制。方法:对SMMU-LTNM肝癌裸鼠皮下模型进行2%浓度的氢氧化钠溶液瘤内注射,检测肿瘤组织微血管密度、HIF-1α和VEGF的表达情况。结果:与生理盐水瘤内注射组相比,氢氧化钠显著抑制肿瘤生长(P<0.01)、降低肿瘤微血管密度(P=0.01)、抑制肿瘤组织HIF-1α和VEGF的表达(P=0.02和P=0.01)。结论:氢氧化钠瘤内注射可有效抑制肝癌生长,主要机制可能是抗血管生成作用。

Objective：To investigate the effects of intratumoral injection of sodium hydroxide on the hepatocellular carcinoma and to investigate the mechanism.Methods：Nude mice bearing subcutaneous SMMU-LTNM tumors were given intratumoral injection with 2% sodium hydroxide or normal saline groups（n=10 for each group）.The inhibitive effects on tumor growth and tumor microvessel density,HIF-1α and VEGF expression were detected.Results：Sodium hydroxide could significantly inhibit tumor growth（P0.01）.Microvessel density was significantly lower in the sodium hydroxide group（P=0.01）,and the expression of HIF-1α and VEGF was sup-pressed（P=0.02 and P=0.01,respectively）.Conclusion：Intratumoral injection with sodium hydroxide could effectively inhibit tumor growth,the main mechanism of which may be the anti-angiogenic effects.