木瓜中熊果酸提取工艺的优化及含量测定

Optimization of Extraction Process of Ursolic Acid from FRUCTUS CHAENOMELIS and Determination of Its Content

[目的]优化黔北产木瓜(FRUCTUS CHAENOMELIS)熊果酸的提取工艺,并测定熊果酸含量,为木瓜的开发利用及质量控制提供科学依据。[方法]采用正交试验优化木瓜熊果酸的提取工艺,用HPLC法测定熊果酸含量。[结果]木瓜熊果酸的最佳提取工艺为:提取时间30 min,无水乙醇用量为25.0 ml,乙醇浓度为100%,在最佳条件下提取的熊果酸含量为0.324%。熊果酸在0.650～3.250μg/ml范围内,与峰面积呈良好的线性关系(r=0.999 1),熊果酸平均回收率为99.20%,RSD值为0.30%。[结论]优选的提取工艺稳定可行,HPLC法简便、灵敏、准确,该研究为木瓜的开发利用及质量控制提供了科学依据。

[Objective] To optimize the extracting conditions of ursolic acid from FRUCTUS CHAENOMELIS and determine its content,so as to provide scientific evidence for the development,application and quality control of ursolic acid.[Method] The extraction conditions of ursolic acid were optimized by orthogonal test,and content of ursolic acid was determined by HPLC.[Result] The optimal extraction conditions were determined to be： 30 min of extraction duration,25.0 ml of ethanol amount and extraction solvent of absolute ethanol.Under the optimum process,content of ursolic acid was 0.324%.When the concentration of ursolic acid ranged from 0.650 to 3.250 μg/ml,the relationship between ursolic acid concentration and peak area was good（r=0.999 1）.Mean recovery rate of ursolic acid was 99.20% with RSD value of 0.30%.[Conclusion] The optimal extraction conditions were feasible,and the established HPLC method was simple,sensitive and accurate.This study provided scientific evidence for the development,application and quality control of ursolic acid.