青海野生与栽培麻花艽psbA-trnH序列的比较

Comparison of Chloroplast DNA psbA-trnH Sequences between Wild and Cultivated Gentiana straminea Maxim.in Qinghai

[目的]分析野生与栽培麻花艽(Gentiana straminea Maxim.)psbA-trnH序列的差异,为麻花艽的基源鉴定和品质评价提供分子依据。[方法]采用PCR扩增纯化后直接测序的方法,测定野生与栽培麻花艽cpDNA psbA-trnH核苷酸序列并作序列同源性分析。[结果]野生与栽培麻花艽的cpDNA psbA-trnH片段长度分别为316和317 bp,两者之间有4个碱基的变异。[结论]利用psbA-trnH区序列的差异可以鉴别野生与栽培麻花艽。

[Objective]To distinguish Gentiana straminea Maxim.from wild and cultivated plants by comparing chloroplast DNA psbA-trnH sequences,so as to provide a molecular basis for orgin identification and quality evaluation.[Method] cpDNA psbA-trnH sequences of Gentiana straminea Maxim.were amplified by polymerase chain reaction（PCR）,and then sequenced by direct PCR sequencing method for homologous analysis.[Result] The lengths of cpDNA psbA-trnH of wild and cultivated plants were 316 and 317 bp respectively,and there were 4 variable sites.[Conclusion] The nucleotide differences of psbA-trnH regions could be used for distinguishing Gentiana straminea Maxim.from wild and cultivated plants.