菲律宾蛤仔提取物提取条件优化及其体外清除自由基的作用

Research on the Role of the Extract of the Ruditapes philippinarum in the Free Radical-scavenging

[目的]研究菲律宾蛤仔全脏器提取物体外对自由基的清除作用,为其综合利用提供基础。[方法]分别采用邻二氮菲-Fe2+氧化法、DPPH反应法和邻苯三酚自氧化法,检测菲律宾蛤仔提取物体外对羟自由基(HO.)、DPPH.和超氧阴离子自由基(O2-.)的清除作用。[结果]以0.1 mol/L NaCl溶液为浸提液,料液比为1∶8,75℃水浴浸提100 min,提取液经透析浓缩,再以70%乙醇终浓度沉淀,提取物(RPS-0)的得率达到3.30%,总糖与可溶性蛋白含量分别为42.40%和14.40%。RPS-0对HO.和DPPH.清除作用的IC50分别为3.100、3.500 mg/ml,对O 2-.的清除率可达到39.4%。[结论]菲律宾蛤仔盐提取物具有一定的体外清除自由基作用。

[Objective]The technical basis of comprehensive utilization of the extracts from the all organs Ruditapes philippinarum for the free radical-scavenging in vitro was provided.[Method]The effect of the extracts from the Ruditapes philippinarum on the scavenging of on hydroxyl the radical（HO·）,the DPPH· radical and superoxide anion free radicals（O2-·） was measured with the method of phenanthroline respectively-Fe2＋ oxidation,DPPH reaction and pyrogallol autoxidation.[Result]The rate of the extract（RPS-0） was 3.30%,the content of total sugar and soluble protein was 42.40% and 14.40% while the NaCl of 0.1 mol/L was as extraction solution,the ratio solid to liquid was 1∶8,75 ℃ and the time of water-extracting was 100 minutes and then the extract solution was concentrated by dialysis concentrated and precipitated with the ethanol with the concentration of 70%.The IC50（the scavenging efficiency of RPS-0 on HO· and DPPH· was 3.100 and 3.500 mg/ml and the removal rate to O2-· reached 39.4%.[Conclusion]The salt extract from Manila clam had some role in free radical-scavenging.