马尾松嫩茎愈伤组织保持、增殖与不定芽分化培养

Callus Maintenance,Proliferation and Differentiation of New Sprout in Pinus massoniana L

笔者以马尾松嫩茎诱导产生的愈伤组织为材料,针对愈伤组织保持、增殖和分化培养中容易出现褐化死亡的现象,探讨培养基中改变肌醇浓度引起的渗透压变化,调节激素配比和改变培养环境对愈伤组织保持、增殖和丛芽分化的影响。结果表明:(1)改良GD培养基,添加肌醇3.0g/L,能有效地解决在愈伤组织增殖培养中的褐化问题;(2)在DCR培养基中添加KT1.0～2.0mg/L和NAA0.5mg/L能逐渐把增殖培养的马尾松愈伤组织从暗培养过渡到光培养,并保持增殖;(3)DCR培养基中添加KT1.0mg/L和TDZ0.5mg/L可以促进愈伤组织向丛生芽分化,并获得4个丛生芽。本研究结果为马尾松优良基因型快繁中愈伤组织保持、增殖和分化培养提供了研究基础。

The author described an adventive bud information from callus by new sproutin vitro propagation of adult Pinus massoniana Lamb.Emphasizing on the callus maintenance,proliferation and differentiation,experiments were conducted to discuss osmotic pressure by inositol,hormone in the medium,as well as culture conditions.The results were as followed：（1）adding inositol 3.0 g/L in the modified GD medium could effectively solve the browning problem and maintaining the callus proliferation;（2）To switch the callus culture condition from dark into light environment,adding KT 1.0-2.0 mg/L and NAA 0.5 mg/L in the DCR medium was viable;（3）DCR medium with KT 1.0 mg/L and thidiazuron（TDZ）0.5 mg/L could promote the callus differentiate into adventive bud and 4 buds achieved.This protocal provided foundation for establishing a system of micropropagation with the superior genotype ofPinus massoniana L.