嫁接对东北山樱(Cerasus sachalinensis Kom.)根际微生物群落结构及多样性的影响

Effect of Grafting on Rhizosphere Soil Microbial Community Structure and Species Diversity of Cerasus sachalinensis Kom.

在植物根际生态系统中,土壤微生物的作用不可忽视,栽培措施的改变会影响根际微生物群落结构,进而影响植物生长。野生条件下,东北山樱嫁接后几十年表现良好,与栽培条件下差异显著。为此,研究野生条件下嫁接对东北山樱根际微生物群落结构以及多样性的影响,对改善樱桃栽培环境及可持续发展具有重要意义。笔者利用选择性培养基,研究野生条件下嫁接与砧木东北山樱根际细菌、真菌、放线菌的数量、群落组成以及种群多样性。结果表明,嫁接对东北山樱根际微生物数量、群落结构及多样性影响差异显著。嫁接显著增加东北山樱根际微生物数量,并形成了以细菌芽孢杆菌属、固氮菌属;真菌木霉菌属、毛霉菌属;放线菌(链霉菌属)吸水类群、烬灰类群和青色类群为主的优势菌属;发现特有细菌埃希氏菌属、节杆菌属、不动杆菌属、德克斯氏菌属、葡萄球菌属。

The importance of soil microorganisms can not be ignored in the rhizosphere ecosystem.Change of cultivation measures will affect community structure of rhizosphere,thereby affecting plant growth.Cerasus sachalinensis Kom.showed good performance after grafting for several decades under wild conditions,and Cerasus sachalinensis Kom.was significantly different under cultivation conditions.Therefore,research of the effect of grafting on rhizosphere soil microbial community structure and species diversity of Cerasus sachalinensis Kom.under wild conditions is important to improve the cultural environment and ststainable development of cherry.In this paper,number of rhizosphere bacteria,fungi and actinomycetes,community composition and diversity of the population were studied under the conditions of grafting and the rootstock of Cerasus sachalinensis Kom.in selective medium.The results showed that the effect of grafing Cerasus sachalinensis Kom.was significantly different.Grafing significantly increased number of rhizosphere microorganisms and the formation ofBacillus,Azotobacter,groups ofHygroscopicus,Cinereus,Glaucuc of streptomyces as dominant species groups based;special bacterial genus Escherichia,Arthrobacter,Acinetobacter,Derxi,Staphylococcus found.