番茄晚疫病病原菌的菌落生长和产孢子条件的研究

Research on Late Blight Disease Germ Colony Growth and Sporulation Conditions of Tomato

为了提高番茄晚疫病病原菌的菌落生长速度和产孢子数量,用佳木斯地区番茄晚疫病植株上分离、纯化的晚疫病病原菌,分别在不同培养基、pH、糖浓度、温度条件下培养,研究番茄晚疫病病原菌菌落生长和产孢子的最佳培养条件。结果表明,黑麦培养基最适宜菌落生长及产孢,最适菌落生长pH值为6.0～7.0,最适产孢子pH值为6.5,孢子生长的最适温度为20℃,培养基中添加蔗糖80g/L时菌落生长快,产孢量大。

In order to improve tomato late blight pathogen colony growth rate and spore production quantities,this research cultured the separated,purificated late blight pathogen from Jamusi local tomato late blight plants,respectively in different media,pH,sugar concentration,temperature conditions,then researched tomato late blight pathogen growth and the best yielding the spore colonies conditions.The results showed that the rye culture medium was suitable for the colony growth and sporulation,the most suitable pH value for the colony growth was 6.0-7.0,the most suitable pH value for the sporulation was 6.5,and the optimum temperature for spore growth was 20℃.When the medium contained 80 g/L sucrose,the colony grew fastest and sporulation was also the greatest.