氮肥施用量对嵌合体‘金旗’玉簪叶色的影响

Effects of nitrogen fertilizer rate on leaf color of chimera Hosta 'Gold standard'

以嵌合体花叶玉簪代表品种‘金旗’为试验材料,研究了4个水平的氮肥处理(盆栽土尿素用量0,1,2,3g/kg)对其不同叶色部位叶色值、叶绿素含量、全氮含量、氮代谢关键酶活性以及叶绿体超微结构的影响。研究结果表明,氮肥主要影响‘金旗’玉簪叶片黄色部位的叶色变化,0和1 g/kg氮肥处理能够维持其花叶性状,叶绿素、全氮含量以及氮代谢关键酶活性等指标无显著变化,叶绿体结构发育不完全,缺乏基粒堆积;2和3 g/kg氮肥处理导致叶片黄色部位转绿,与0和1 g/kg氮肥处理下各指标相比,叶绿素、全氮含量显著提高,谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)活性显著上升,叶绿体超微结构发育完全,并且出现大量类囊体片层结构。

The effects of four nitrogen levels（0,1,2,3 g/kg soil） on leaf color,chlorophyll contents,total N contents,key enzymes activities of nitrogen metabolism and chloroplast ultrastructure of chimera Hosta ＇Gold standard＇ were studied.Nitrogen levels mainly influenced the colour of the yellow leaf area of Hosta ＇Gold standard＇.The chimera characteristics were maintained under 0 and 1 g/kg nitrogen levels.There were no significant differences in chlorophyll contents,total N contents,and activities of key enzymes of nitrogen metabolism.The formation of chloroplasts which lack normal grana stacking was severely inhibited in the yellow leaf area.Under 2 and 3 g/kg nitrogen levels,the yellow leaf area became green.Compared with the indexes under 0 and 1 g/kg nitrogen levels,chlorophyll contents,total N contents and activities of key enzymes of nitrogen metabolism were significantly increased.Chloroplast ultrastructure was fully developed and normal thylakoid lamellar structure was formed.