3,4,5-三羟基苯甲酸印迹聚合物的分子识别特性

Molecular Recognition Performances of 3,4,5-Trihydroxybenzoic Acid-Imprinted Polymer

采用非共价印迹技术,以3,4,5-三羟基苯甲酸(3,4,5-THBA)为印迹分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,乙腈为致孔剂,60℃热引发制备了印迹聚合物P(3,4,5-THBA),用高效液相色谱法和Scatchard作图评价了其选择性结合特性。结果表明,P(3,4,5-THBA)对3,4,5-THBA具有选择性结合能力,氢键结合在其分子识别过程中起主要作用,其中存在两类结合位点,高亲和力结合位点的离解常数KD1=2.4×10-4mol/L,最大结合位点数Qmax1=30.6μmol/g;低亲合力结合位点的离解常数KD2=2.3×10-3mol/L,最大结合位点数Qmax2=91.1μmol/g。

3,4,5-Trihydroxybenzoic acid（3,4,5-THBA）-imprinted polymer P（3,4,5-THBA） was synthesized thermally initiated at 60℃ by non-covalent molecular imprinting technique using 3,4,5-THBA as template molecule,acrylamide as functional monomer,ethyleneglycol dimethacrylate as cross-linker,and acetonitrile as porogenic solvent.The specific rebinding characteristics of P（3,4,5-THBA） for 3,4,5-THBA were investigated via high-performance liquid chromatography and Scatchard analysis.It was revealed that P（3,4,5-THBA） showed high specific affinity to 3,4,5-THBA and hydrogen bonding played a major role and that two kinds of sites existed on the polymer matrix with their dissociation constants being estimated to be 2.4×10-4mol/L and 2.3×10-3mol/L,respectively,and the binding sites being 30.6μmol/g and 91.1μmol/g,respectively.