HPLC法同时测定大籽獐牙菜中10种化学成分

Simultaneous Determination of 10 Chemical Components in Large Swertia Seeds by HPLC

建立反相高效液相色谱法同时测定大籽獐牙莱中10种成分的含量。方法：采用反相高效液相色谱，色谱条件为：AgilentXDB C18柱（中4．6×150mm，5mm）；流动相A：甲醇，B：水；洗脱方法：0～20min，A为10％～30％，20～45minA为30％～80％梯度洗脱；体积流量1．0mL／min，检测波长254nm，230nm，柱温28℃。结果：10种成分均达到基线分离，各化合物的线性关系良好，r〉0．9995；各待测物的精密度RSD值均小于1．1％；重复性良好（RSD〈3．7％）；回收率范围在97．8％～104．2％，RSD〈C4．2％。结论：该方法测定成分多，结果准确、可靠。

Objective:To establish a RP-HPLC method for simultaneous determination of 10 chemical components in large Swertia seeds.Methods: RP-HPLC was used,with chromatographic conditions: agilent XDB C18 column(Φ4.6×150 mm,5 μm);mobile phase A: methanol,B: water;elution: 0~20 min;gradient elution,A: 10%~30%,20~45 min A: 30% to 80%;volume flow rate 1.0 mL/min,detection wavelength 254 nm,230 nm;column temperature 28 ℃.Results: 10 components were baseline separated,with a good linear relationship with the compounds,r>0.999 5;the precision of the analytes was less than 1.1% RSD,with a good reproducibility(RSD<3.7%);recovery at the range of 97.8%~104.2%,RSD<4.2%.Conclusion: The method can determine more components and the results are accurate and reliable.