盐酸小檗碱对G6PD酶缺陷患者红细胞的影响

Berberine hydrochloride on G6PD enzyme defects in red blood cells of patients

目的本文研究盐酸小檗碱是否会诱发红细胞G6PD酶活性缺陷患者发生溶血,为G6PD酶缺陷患者使用黄连的安全性提供参考。方法采用G6PD酶缺陷患者静脉全血,以生理盐水为空白对照,观察不同浓度盐酸小檗碱(Ber)对红细胞形态、红细胞计数(RBC)、血红蛋白(HGB)、红细胞G6PD酶活性及血浆游离血红蛋白(F-HB)的影响。结果血中Ber浓度为0.1mg/L和0.5mg/L时,红细胞形态、RBC、HGB及G6PD酶活性均无明显改变(P>0.05);Ber药物浓度为1mg/L时,红细胞形态有轻度改变,RBC、HGB下降(P<0.05),G6PD酶活性无明显变化(P>0.05);Ber浓度为5mg/L时,红细胞形态明显改变,RBC、HGB显著下降(P<0.01),红细胞G6PD酶活性显著增高(P<0.01);各浓度Ber溶液均可使F-HB显著下降(P<0.01)。结论①按正常剂量使用黄连对G6PD酶缺乏者可能是安全的,大剂量Ber能使G6PD酶缺陷患者红细胞遭到损害,提示避免超剂量使用黄连。②Ber能使血浆游离血红蛋白测定值降低,可能的原因有待进一步研究分析。

Objective： This paper aims to study whether berberine can induce hemolysis for patients that have defects of G6PD activity in red blood cell,and provide a reference for patients with G6PD enzyme defects.Methods：Firstly,take whole blood from patients with G6PD defects,and then Observe the impact of different concentrations of berberine（Ber） on erythrocyte morphology,red blood cell count（RBC）,hemoglobin（HGB）,erythrocyte G6PD activity and plasma free hemoglobin（F-HB）,at the same time,take Hydrochloric acid as blank control group to be compared with.Results： The blood concentration of berberine 0.1mg / L and 0.5mg / L,the red blood cell morphology,RBC,HGB and G6PD activity had no significant changes（P〉0.05）;berberine concentration of 1mg of drug solution / L,had mild changes in red blood cell morphology,RBC,HGB decreased（P〈0.05）,G6PD activity had no significant changes（P〉0.05）;berberine concentration is 5mg / L,the red blood cell morphological had sinifcant changes,RBC,HGB decreased significantly（P〈0.01）,red blood cell G6PD activity was significantly higher（P〈0.01）;the concentration of berberine solution to F-HB can be significantly decreased（P〈0.01）.Conclusion：①Coptis the normal dose of G6PD enzyme deficiency may be safe,large doses can damage Ber G6PD erythrocyte enzyme deficiencies,reminding to avoid the use of berberine overdose.②berberine can reduce plasma free hemoglobin values,the possible reasons for further research and analysis.