何首乌饮对衰老大鼠卵巢细胞凋亡的影响

Effect of polygonum multiflorum drink on apoptosis of ovarian cells in aging rats

目的:探讨何首乌饮对亚急性衰老大鼠卵巢细胞凋亡相关基因P53和Bcl-2/Bax蛋白表达的影响。方法:采用D-半乳糖连续腹腔注射致亚急性衰老大鼠模型,在造模后以何首乌饮灌胃作为延缓衰老组;造模后应用逆转录聚合酶链反应测定各组P53的mRNA水平;Western Bloting法检测卵巢细胞Bcl-2和Bax f蛋白的表达情况。结果:与正常对照组比较,D-半乳糖致衰老的模型组大鼠卵巢细胞中P53基因和Bax蛋白表达明显上升,Bcl-2蛋白表达显著下降,差异有统计学意义(P<0.01);何首乌饮延缓衰老组较模型组P53基因和Bax蛋白表达明显下降(P<0.01),并上调Bcl-2蛋白的表达,呈现量效关系,以何首乌饮高剂量组效果作用最佳。结论:何首乌饮方剂可能是通过调节卵巢细胞凋亡相关基因和蛋白表达,来延缓衰老大鼠卵巢细胞凋亡的发生。

Objective：To explore the effect of polygonum multiflorum drink on expressions of apoptosis related gene-p53 and Bcl-2/Bax protein of ovarian cells in subacute aging rats.Methods：The rat models of subacute aging were established by intraperitoneal injection of D-gal,the rats treated with pouring polygonum multiflorum drink into stomach were selected as deferring senescence group;RT-PCR was used to detect the expression levels of p53 mRNA in all groups;Western Bloting was used to detect the expression levels of Bcl-2 protein and Bax protein in ovarian cells.Results：Compared with normal control group,the expression levels of p53 gene and Bax protein in ovarian cells of D-gal induced aging rat model group increased significantly,the expression level of Bcl-2 protein decreased significantly（P0.01）;the expression levels of p53 gene and Bax protein in ovarian cells of deferring senescence group decreased significantly（P0.01）,the expression level of Bcl-2 protein increased,showing a dose-effect relationship,the effect of polygonum multiflorum drink of high dose was the best.Conclusion：Polygonum multiflorum drink may delay the apoptosis of ovarian cells in aging rats by regulating the expressions of apoptosis related gene and protein in ovarian cells.