消旋-3-正丁基苯酞对血管性痴呆大鼠学习记忆能力的影响

The effect of 3-n-butylphthalide on learning and memory abilities in vascular dementia rats

目的 探讨消旋-3-正丁基苯酞（NBP）对血管性痴呆大鼠学习记忆的影响.方法 用两血管法（2VO）建立血管性痴呆（vascular dementia,VD）模型,将60只3月龄雄性Wistar大鼠随机分成VD组、假手术组和NBP组,NBP组以丁苯酞灌胃1个月,VD组和假手术组给予等量的植物油,以Morris水迷宫测试各组大鼠的学习记忆能力,HE染色观察大鼠海马区脑组织形态.结果 NBP组和假手术组和VD组的隐蔽平台逃避潜伏期[分别为（38.34±2.46）s,（14.83±3.77s）,（75.74±6.33）s];原平台象限时间[分别为（26.45±4.66）s,（35.21±3.78）s,（18.67±5.39）s].与VD组相比,NBP组明显缩短隐蔽平台逃避潜伏期,增加原平台象限时间,并在HE染色中减少神经元坏死.结论 NBP能显著改善VD大鼠的学习记忆能力.

Objective To investigate the effect of 3-n-butylphthalide （NBP） on learning and memory abilities in vascular demensia （VD） rats. Methods VD model rats was established by two-vessel method. 60 3-month-old Wistar male rats were randomly divided into VD group, sham-operate group and NBP group. Rats in NBP group were given NBP 120 mg · kg-1 · d-1 ,VD and sham-operate group were given equal quality vegetable oil.Morris water maze was used to assess the learning and memory abilities in each group and HE staining was used to observe the hippocampus morphology of the rats. Results The escape latency in hidden plat test was（ （38.34 ±2.46 ） s, （ 14.83 ± 3.77s ）, （ 75.74 ± 6.33 ） s ） and the original platform quadrant time was （ （ 26.45 ± 4.66 ） s,（35.21 ±3.78）s, （ 18.67 ±5.39）s） in NBP group,sham-operate group and VD group respectively. Compared to VD group, NBP group had obviously decreased escape latency in hidden plat test, increased original platform quadrant time and distinctly decreased the necrosis of the neurons in HE dyeing. Conclusion NBP can improve the learning and memory deficits in VD rats.