慢性淋巴细胞白血病检测t(11;14)、CD5和CD23的诊断价值

The role of detecting t(11;14),CD5 and CD23 in diagnosis of chronic lymphocytic leukemia

目的:探讨慢性淋巴细胞白血病(CLL)检测t(11;14)、CD5和CD23的诊断价值。方法:采用流式细胞术(FCM)和常规细胞遗传学(CC)对45例初诊为CLL的患者进行免疫学和细胞遗传学检测,并采用间期荧光原位杂交(FISH)技术进行t(11;14)的研究。结果:45例初诊CLL中,CC均未见t(11;14)异常,FCM示5例CD5+CD23-,40例CD5+CD23+。5例CD5+CD23-患者中,4例经I-FISH检测出具有t(11;14)异常。40例CD5+CD23+患者中,I-FISH检测无一例有t(11;14)异常。结论:t(11;14)、CD5和CD23在CLL诊断中具有重要意义,CLL免疫表型多为CD5+CD23+,且无t(11;14)异常。少数套细胞淋巴瘤(MCL)误诊为CLL,MCL多有t(11;14)异常,免疫表型为CD5+CD23-。

Objective：To investigate the role of detecting t（11;14）,CD5 and CD23 in diagnosis of chronic lymphocytic leukemia（CLL）.Method：Flow cytometry（FCM） and conventional cytogenetics （CC） were performed in 45 patients who were firstly diagnosed as CLL,then interval fluorescence in situ hybridization（I-FISH） was performed in all patients.Result：All patients were negative with t（11;14） in CC.FCM results showed that 5 patients were CD5＋CD23-and 40 patients were CD5＋CD23＋.Four patients with CD5＋CD23-were positive with t（11;14） by I-FISH,but no patients with CD5＋CD23＋ were positive with t（11;14） by I-FISH.Conclusion：Examination of t（11;14）,CD5 and CD23 plays an important role in diagnosing CLL.CLL shows features of t（11;14） negative and CD5＋ CD23＋.A few of mantle cell lymphoma（MCL） cases are misdiagnosed as CLL. MCL is identified as t（11;14） and CD5＋ CD23-.