人免疫缺陷病毒2型(HIV-2)穿膜蛋白基因片段克隆表达

MOLECULAR CLONING AND EXPRESSION OF GENE FRAGMENT CODING FOR A DOMINANT EPITOPE OF HUMAN IMMUNODEFICIENCY VIRUS 2 IN E.COLI

以人免疫缺陷病毒２（ Ｈ Ｉ Ｖ２） 的原病毒基因组为模板，通过套式 Ｐ Ｃ Ｒ 扩增得到了 Ｈ Ｉ Ｖ２ 的一段特异性膜抗原蛋白基因片段；随后将此片段克隆入诱导表达载体ｐ Ｇ Ｅ Ｘ５ Ｘ１ ，获得了重组表达质粒ｐ Ｇ Ｅ Ｘ３６ Ｅ Ｎ Ｖ． Ｉ Ｐ Ｔ Ｇ 对重组表达菌株诱导后， Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ

A DNA fragment coding for a dominant epitope of the transmembrane region from Human Immunodeficiency Virus2(HIV 2) Proviral genome was amplified by nested PCR,which was directly cloned into pGEM R T vector.The following enzymatic analysis primarily verified the amplified product.The DNA fragment was then inserted into pGEX 5X 1 expression plasmid and the recombinant plasmid pGEX36 ENV wsa transformed into E.coli.The result of SDS PAGE analysis showed that a new protein band corresponding to the fusion form between GST and the objective peptide was produced.