Tb(Ⅲ)荧光探针测定肾上腺素

TERBIUM(Ⅲ)AS FLUORESCENCE PROBE FOR THE DETERMINATION OF EPINEPHRINE

肾上腺素（ Ｅ） 与 Ｔｂ３ ＋ 在ｐ Ｈ ６ ．６ ～７ ．２ 条件下能形成较强的荧光络合物，其激发峰在３０５ ｎ ｍ ，发射峰在４９４ ｎ ｍ 和５４５ ｎ ｍ 处，肾上腺素在８ ．２ ×１０ － ８ ～９ ．８ ×１０ － ５ ｍｏｌ／ Ｌ 范围内与其荧光强度呈线性关系，检出限为２ ．２５ ×１０ － ８ ｍｏｌ／ Ｌ．

It has been found that epinephrine(E) can form complex with Tb(Ⅲ) at pH6.6～7.2,which emits characteristic fluorescence of Tb(Ⅲ).The excitation and emission wavelengths are 305 nm,494 nm and 545 nm,respectively.A linear reltationship has been obtained between the fluorecense intensity and E concentration in the range of 8.2×10 -8 ～9.8×10 -5 mol/L The detection limit is 2.25×10 -8 mol/L(S/N=3).The determination of samples give satisfactory results.