粘虫血淋巴凝集素的性质研究

Isolation And Characterization of A Lectin From The Hemolymph of The Mythimmnia Separata

粘虫（（Mythimmniaseparata）血淋巴对人ABO血型系统红细胞有同等的凝集活性，对兔、小白鼠、豚鼠、蟾蜍、鲫鱼、鸡红细胞有不同的凝集活性，对山羊红细胞无凝集活性．其凝集活性能被D—半乳精明显地抑制．对Ca2＋无依赖性．70℃处理15min凝集活性趋于零．粘虫核型多角体病毒的诱导使其活性明显增加．化蛹早期及蛹期凝集活性下降．血淋巴凝集素通过盐析、凝胶过滤和纤维素层析得到分离．它在SDS—PAGE中表现为单一带，为糖蛋白，分子量为6．08×104．其蛋白质含量为96．74％，糖含量为1．36％．

The lectin from the hemolymph of Mythimmnia separata Larvae was found to have the same activity to agglutinate the red blood cells of human type A, B and O. It could not agglutinat the red blood cells of sheep. This activity was inhibited distinctly by D-galactose and was independent of Ca2+. When the hemolymph was heated at 70℃ for 15 min, its aggluginating activity almost disappeared. The activity was greatly increased by the induction of HaNPV. The lectin was isolated by (NH4)2SO4 salting out, SephadeX G-100 gel filtration and DEAE-PAGE. The molecular-weingt of the lectin was 6. 08×104. It was of glucoprotein. It contains 96. 74% protein and 1. 36 % carbohydrate.