棘孢小单胞菌原生质体的融合育种

STUDY OF PROTOPLAST FUSION OF MICROMONOSPORA ECHINOSPORA PRODUCING STRAIN

以小诺霉素（Ｍｉｃｒｏｎｏｍｉｃｉｎ，ＭＣＲ）产生棘孢小单胞菌（Ｍｉｃｒｏｍｏｎｏｓｐｏｒａｅｃｈｉｎｏｓｐｏｒａ）为出发菌株，诱变筛选得到一株链霉素抗性菌株，抗性菌株的原生质体分别用紫外线照射和热处理致死，通过单亲致死原生质体融合，以链霉素为选择条件选出融合株，经摇瓶发酵并结合薄层层析扫描（ＴｈｉｎＬａｙｅｒＣｈｒｏｍａｔｏｇｒａｐｈｙ，ＴＬＣ）分析，筛得４株ＭＣＲ百分含量高于亲株的融合子，ＭＣＲ百分含量达到９０％，效价１０７６ｕ／ｍｌ，分别比亲株提高１５％和１１％，

Micromonospora echinospora , a micronomicin producer,was mutated then screened a streptomicin resistance strain.The fusion bodies were screened with the protoplast of single parent strain inactivation by U.V and heat,respectively.Four strains of MCR percentage being higher than their parent by fermentation and thin layer Charomatography analysis are gotten.The MCR percentage and titer dre higher than their parent strains,15% and 11% respectively.