STGC3基因缺失突变对CNE2细胞生长增殖能力的影响

Effect of STGC3 Gene Deletion Mutant on The Growth of CNE2 Cells

为探讨鼻咽癌候选抑癌基因STGC3中层粘连蛋白G结构域(LG domain)对CNE2生长增殖能力的影响,应用基因定点突变技术将该结构域缺失,亚克隆至真核表达载体上,并将野生型及突变型STGC3稳定转染人鼻咽癌细胞系CNE2,检测其对CNE2细胞系表型的影响,包括测定稳转细胞系的生长曲线、细胞集落形成能力和细胞周期分布.研究发现,LGdomain的缺失明显降低了STGC3的肿瘤抑制活性,使受其稳定转染的细胞系恶性度显著增强,提示该结构域在STGC3发挥肿瘤抑制功能中起着重要作用.

To investigate the function of Laminin G domain （LG domain） of STGC3 in human nasopharyngeal carcinoma cell line CNE2. Recombinant plasmids pcDNA3.1 （＋）-STGC3 and pcDNA3.1 （＋）-STGC3△1 -42AA were respectively transfected into CNE2 cells by liposome-mediated transfection. Therefore, the CNE2/pcDNA3.1 （＋）- STGC3 and CNE2/pcDNA3.1 （＋）-STGC3△1-42AA cell lines of stable expression STGC3 were established. Here, it is reported that deletion of LG domain in this STGC3 reduces the tumor suppression activity of it, as demonstrated by drawing growth curve, experimenting plate clone formation as well as detecting cell cycle distribution. The results showed： the ability of STGC3△1-42AA suppressing CNE2 cell proliferation was obviously decreased to compare with wild type STGC3 （n=4, P 〈 0.05）. These results indicate that the LG domain is necessary for the tumor suppression activity of the STGC3.