筛选蛋白质结晶条件的试剂盒研发进展

Progresses on Developing Screening Kits for Protein Crystallization

蛋白质结晶条件筛选是蛋白质分子三维结构解析的主要限速环节之一,因此发展筛选效率较高的结晶条件筛选试剂盒有着十分重要的意义.目前广泛应用的结晶条件筛选试剂盒的设计方法主要有不完全因子法、稀疏矩阵法和系统筛选法.结晶学家基于这三种方法研发了一系列用于蛋白质结晶条件筛选的试剂盒.评述了20世纪以来筛选试剂盒的设计方法及应用进展,并展望了其未来的发展方向.

Screening of protein crystallization conditions remains the major limiting step for three-dimensional structure determination, therefore developing more efficient screening kits is essential. Up to now the methods used for designing the screening kits include the incomplete factorial design, the sparse matrix sampling and the systematic screening. Based on these three methods, researchers have developed a series of crystallization screening kits which are proved applicable in practical protein crystallization. The development in designing the screening kits and the applications of the screening kits since 20th century were reviewed, and the future prospect in this field was discussed.