51例安徽汉族免疫性不育症患者与HLA-DQA1基因相关性及免不Ⅲ号的治疗

Study of the Correlation between 51 Immune Infertility Patients of the Han Nationality in Anhui and HLA-DQA1 Gene and Treatment of Mianbu Ⅲ

目的探讨抗精子抗体阳性的免疫性不育症患者与人类白细胞抗原-DQA1(human leucocyte anti-gen-DQA1,HLA-DQA1)基因的相关性及中医药治疗与HLA-DQA1基因型的关系。方法 采用聚合酶链反应-序列特异性引物(polymerase chain reaction-sequence specific primer,PCR-SSP)技术,对51例抗精子抗体阳性的免疫性不育症患者和60名正常健康人的HLA-DQA1基因进行分型研究,并对患者用中医免疫方免不Ⅲ号(生地、白芍、山萸肉、山药、枸杞子、丹皮、麦冬)治疗3个月。结果 免疫性不育症组HLA-DQA1*0401等位基因频率明显高于正常对照组(χ2=29.869,P<0.01),免不Ⅲ治疗后,27例HLA-DQA1*0401转阴22例(81.5%),差异有统计学意义(χ2=5.24,P=0.022)。结论 HLA-DQA1*0401等位基因可能是抗精子抗体阳性的免疫性不育症的易感基因,HLA-DQA1*0401等位基因的患者经中药治疗有效。

Objective To explore the correlation between anti-sperm antibody-positive immune infertility patients and human leucocyte antigen（HLA） DQA1 gene,and to study the correlation between the treatment of Chinese medicine and pharmacy and HLA-DQA1 genotype.Methods The polymerase chain reaction-sequence specific primers（PCR-SSP） technique was used in studying HLA-DQA1 genotypes of 51 anti-sperm antibody-positive immune infertility patients and 60 healthy subjects.MianbuⅢ（consisting of rehmannia root,white peony root,Fructus Corni,yam,barbary wolfberry fruit,moutan bark,and dwarf lilyturf root） was used in patients for three months.Results The HLA-DQA10401 allele in the immune infertility group was obviously higher than that in the healthy control group（χ2=29.869,P0.01）.As for the therapeutic efficacy 22 cases of the 27 positive HLA-DQA10401 turned to negative with statistical difference（χ2=5.24,P=0.022）.ConclusionHLA-DQA10401 allele might be predisposing gene of the anti-sperm antibody-positive immune infertility.The Chinese medicinal treatment was effective for the HLA-DQA10401 allele patients.