加味丹寿汤对双酚A染毒孕鼠之胎鼠致畸的防护作用

Protective Effect of Modified Danshou Decoction on Teratogenicity of Bisphenol A Intoxicated Pregnant Rats

目的探讨加味丹寿汤对双酚A染毒孕鼠之胎鼠致畸的防护作用。方法 将44只受孕大鼠随机分为4组,空白对照组与模型组各10只,加味丹寿汤组与寿胎丸组各12只。模型组、加味丹寿汤组及寿胎丸组自孕第1～20天均予双酚A600mg/kg灌胃,空白对照组予大豆油灌胃。其间模型组不予其他干预,其他3组分别予生理盐水、加味丹寿汤及寿胎丸浓煎液灌胃。所有孕鼠均于孕20天处死取材。结果 与模型组比较,加味丹寿汤能增加孕鼠及胎鼠体质量,改善胎鼠身长及尾长(P<0.05),且优于寿胎丸(P<0.05)。加味丹寿汤还可降低胎鼠畸形率(P<0.05)。结论 加味丹寿汤能促进孕鼠体质量增长,改善胎鼠体质量、身长及尾长,降低胎鼠畸形率。

Objective To explore the protective effect of Modified Danshou Decoction（MDD） on teratogenicity of bisphenol A intoxicated pregnant rats.Methods Forty-four successfully mated rats were randomly divided into 4 groups,10 in the blank group and 10 in the model group,12 in the MDD group and 12 in the positive control group.Bisphenol A（BPA） at the dose of 600 mg/kg was given to rats by gastrogavage in the latter three groups from the 1st day of mating to the 20th day,while the soybean oil was given to rats by gastrogavage in the blank group.No intervention was given to rats in the model group,but the normal saline,MDD condensed decoction,and Shoutai Pill（STP） condensed decoction was respectively given to rats in the rest three groups during the experimental period.All rats were sacrificed by the 20th pregnancy day.Results Compared with the model group,the body weight of pregnant rats and fetal rats,body length and tail length of the fetal rats significantly increased in the MDD group（P0.05）.But the effect of MDD was superior to that of STP（P0.05）.Moreover,the teratogenic rate was significantly lowered in the MDD group（P0.05）.Conclusion MDD could promote the weight gaining of pregnant rats and fetal rats,improve the body length and tail length of fetal rats,and lower the teratogenic rate in fetal mice.