滋阴补阳序贯法对多囊卵巢综合征大鼠血清性激素水平的影响

Effect of Sequential Method of Nourishing Yin and Tonifying Yang on Serum Sex Hormone Levels in Rats with Polycystic Ovary Syndrome

目的观察滋阴补阳序贯法对多囊卵巢综合征(PCOS)模型大鼠血清性激素水平的影响,并探讨其可能的作用机制。方法 采用皮下注射脱氢表雄酮(DHEA)造模法建立PCOS大鼠模型。将70只21日龄SD雌性大鼠随机分为7组(每组10只):正常对照组、模型组、滋阴组、补阳组、序贯组、西药组及联合用药(序贯加西药)组。各组连续灌胃12天。采用化学发光法检测血清睾酮(T)、雌二醇(E2)水平,放射免疫法(RIA)检测血清雄烯二酮(A)、促卵泡激素(FSH)、黄体生成素(LH)、胰岛素(INS)和胰岛素样生长因子Ⅰ(IGF-Ⅰ)水平。结果 模型组血清A、T、E2水平较正常对照组明显升高(P<0.01,P<0.05);序贯组血清A、T及E2水平较模型组明显降低(P<0.01,P<0.05);模型组血清INS、IGF-Ⅰ水平较正常对照组明显升高(P<0.05);序贯组血清INS、IGF-Ⅰ水平明显低于模型组(P<0.01)。结论 滋阴补阳序贯法能够降低PCOS大鼠血清A、T、E2水平,并能降低PCOS大鼠血清INS、IGF-Ⅰ水平,逆转其内分泌紊乱状态。滋阴补阳序贯法降低PCOS大鼠血清A、T、E2水平的机制之一可能是通过降低血清INS、IGF-Ⅰ水平。

Objective To observe the effect of sequential method of nourishing yin and tonifying yang on serum sex hormone levels in rats with polycystic ovary syndrome（PCOS）,and to explore its possible mechanisms.Methods PCOS rat models were established by injecting dehydroepiandrosteron（DHEA） subcutaneously.A total of 70 21-day female SD rats were randomly divided into seven groups,ten in each group：the normal control,the model control,the yin nourishing,the yang tonifying,the sequential method,the Western medicine,and the combined medication.Rats in all groups were treated by gastrogavage for twelve successive days.Serum testosterone（T） and estradiol（E2） were detected by chemoluminescence method.Serum androstenedione（A）,follicle stimulating hormone（FSH）,luteinizing hormone（LH）,insulin（INS）,and insulin-like growth factor-Ⅰ（IGF-Ⅰ） were detectd by radioimmunoassay（RIA）.Results Serum levels of A,T,and E2 were significantly higher in the model control group than in the normal control group（P0.05,P0.01）.Serum levels of A and T were obviously lower in the sequential group than in the model control group with significant difference（P0.01）.The serum E2 level was obviously lower in the sequential group than in the model control group（P0.05）.Serum levels of INS and IGF-Ⅰ were significantly higher in the model control group than in the normal control group（P0.05）.Serum levels of INS and IGF-Ⅰ were significantly lower in the sequential group than in the model control group（P0.01）.Conclusions The sequential method of nourishing yin and tonifying yang can lower serum levels of A,T,E2,INS,and IGF-Ⅰ,and reverse its endocrine disturbance in PCOS rats.One of its mechanisms in decreasing serum levels of A,T,E2 might be possibly through lowering serum levels of INS and IGF-Ⅰ.