加速度暴露后发生晕动病与未发生晕动病人群血清微量元素分析

Comparison of serum trace element concentrations between individuals with and without motion sickness after exposure to acceleration

目的分析加速度暴露后发生晕动病与未发生晕动病人群的血清微量元素的变化特点。方法运用六自由度舰船运动模拟装置对60名受试对象进行加速度暴露。根据暴露后是否出现恶心、呕吐症状将受试者分为晕动病组和无晕动病组。采用电感耦合等离子体发射光谱法测定加速度暴露前后两组受试者血清微量元素的含量,采用放免法或高效液相-电化学法检测血液中皮质醇、肾上腺素含量。结果加速度暴露前2组人群所有检测出的微量元素、应激激素含量间差异均没有统计学意义;与自身暴露前相比,暴露后无晕动病组人群血清铜、锶和锰的含量升高,而晕动病组没有统计学差异;晕动病组人群血清锌的含量较暴露前降低了19.7%(P<0.01),而无晕动病组没有明显变化;暴露后晕动病组与无晕动病组的血清铁含量相比降低了17.1%(P<0.05);加速度暴露后所有受试者血清皮质醇和肾上腺素含量均升高,但2组之间差异没有统计学意义。结论加速度暴露造成的血清铜、锶和锰含量上升以及血清锌、铁含量降低可能与晕动病有关。

Objective To analyze the changes of serum trace element concentrations between subjects with and without motion sickness after exposure to acceleration,so as to provide new insights on the etiology and pathogenesis of motion sickness.Methods A total of 60 volunteers were exposed to the repetitive acceleration stimulation using a 6-degree-of-freedom ship motion simulator.At the end of the acceleration exposure the subjects were divided into motion sickness and non-motion sickness groups according to the presence of nausea and vomiting.The serum samples were collected immediately after acceleration exposure to determine the concentrations of trace elements,levels of cortisol and epinephrine by inductively-coupled plasma atomic emission spectrometry（ICP-AES）,radioimmunoassay（RIA） and high-performance liquid chromatography coupled to electrochemical detection（HPLC-ECD）.Results There were no significant differences in trace elements,cortisol and epinephrine concentrations between the motion sickness and non-motion sickness groups before exposure to acceleration.After exposure,serum Cu,Sr and Mn concentrations were apparently increased in the non-motion sickness group and not changed in the motion sickness group.Serum Zn concentration in the motion sickness group decreased by 19.7% after exposure（P〈0.01）,and it did not change in the non-motion sickness group.Serum Zn concentration in the motion sickness group decreased by 17.1% compared with that in the non-motion sickness group after exposure（P〈0.05）.Serum cortisol and epinephrine levels increased in both groups,and there were no significant differences between the two groups.Conclusion Our results suggest that acceleration exposure-induced increase of serum Cu,Sr,Mn,and decrease of Fe,Zn concentrations may be related to the development of motion sickness.